Bishop Noonan led the bilingual and interreligious prayer service at Orlando's St. James Cathedral. Called the "Vigil to Dry Tears," the event drew clergy and a congregation from diverse faiths.
The diocese said the vigil was an opportunity "to bring about an outpouring of the mercy of God within the heart of our community."
Early Sunday morning, a gunman opened fire at a gay nightclub in Orlando and began taking hostages. He killed 49 people and injured 53 more before he himself was killed by SWAT officers responding to the scene.
Scripture readings at the evening vigil included the Prophet Isaiah: "He will destroy death forever. The Lord God will wipe away the tears from all faces." The Gospel of Matthew was also read: "Jesus said come to me, all you who are labored and burdened, and I will give you rest."
The prayer vigil brought together local residents and local leaders including Bishop Robert Lynch of St. Petersburg, an imam from an Orlando Islamic center and several Christian ministers from other denominations.
"We come to pray and in a sense of love and respect for one another, I wanted to bring the whole religious community together," Bishop Noonan said.