"We do not know whether anti-Muslim bias played a role in this crime, but I do recognize the fear that members of our community may feel," Kleinschmidt stated on Wednesday.
President Obama decried the "brutal and outrageous murders" in a Friday statement.
"As we saw with the overwhelming presence at the funeral of these young Americans, we are all one American family. Whenever anyone is taken from us before their time, we remember how they lived their lives – and the words of one of the victims should inspire the way we live ours," he stated.
Outside the White House in below-freezing temperatures, attendees of the prayer service emphasized peace and love in response to the killings which they saw as a religiously-motivated hate crime.
"We didn't know them personally, but they represent each and every one of us Muslims living in the U.S. We're American, we're Muslims living in this country, we give back to our community as much as we can," said participant Miriam Ismail, from Boston.
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"I think it's really important for the general community worldwide to understand that Muslims do represent peace. We represent justice. We represent humanity," she added.
Another participant, Mahmoud, said he was inspired to attend the service by a sister of one of the victims.
"Her speech was magnificent," he said. "It was just so powerful, it carried a lot of wisdom, a lot of love, no hate."